Radix phytolaccae on tissue metabolomics in rats by gas chromatography-mass spectrometry

Traditional Chinese medicine Radix phytolaccae is the dried root of Phytolacca acinosa Roxb and Phytolacca americana L. Radix phytolaccae has multiple efficacies of antibiosis, antivirus, antiinflammatory, antitumor, enhancing immunity, eliminating phlegm and relieving asthma. In this study, we developed a tissue (liver, kidney and heart) metabolomic method by gas chromatography-mass spectrometry (GC-MS) to evaluate the effect of Radix phytolaccae in rats. The rats were divided into three groups, the control group, Radix phytolaccae (Low, 0.5 g/kg) treated group, Radix phytolaccae (High, 1.0 g/kg) treated group. Compared to the control group, L-proline, 9H-purine, myo-inositol, arachidonic acid increased and d-glucose, D-glucopyranoside decreased in liver of the Low group. Compared to the control group, d-glucose decreased in liver of the High group. Compared to the control group, the glycine, myo-inositol increased in kidney of the Low group. Compared to the control group, urea and dmannose increased; L-valine, L-leucine, mannose, L-threonine, L-proline, L-phenylalanine, 9H-purine decreased in kidney of the High group. Compared to the control group, d-mannose and L-tyrosine increased in kidney of the High group. The changes of metabolites increased or decreased, indicating that the effect of Radix phytolaccae could induce energy metabolism, amino acid metabolism, urea cycle perturbations in rats.